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A Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extract

2020-03Journal article Open access
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dc.contributor.authorRosa, Gonçalo P.
dc.contributor.authorBarreto, Maria do Carmo
dc.contributor.authorPinto, Diana C. G. A.
dc.contributor.authorSeca, Ana M. L.
dc.date.accessioned2021-03-25T12:58:45Z
dc.date.available2021-03-25T12:58:45Z
dc.date.issued2020-03
dc.description.abstractRecently there is a great social expectation that scientists should produce more sustainable and environmentally friendly chemical processes. Within this necessity, biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials. In this work, we propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root. The protocol described includes (1) the preparation of the enzymatic extract, (2) the procedure for the assessment of the more favorable working parameters (temperature, pH) and (3) the methodology for the application of the extract as the catalyst for biotransformation reactions. We anticipate that the protocol in this research will provide a simple way for obtaining an enzymatic extract which can operate efficiently under mild conditions and can effectively catalyze the biotransformation of simple phenols.en
dc.description.sponsorshipThis research was funded by FCT—Fundação para a Ciência e a Tecnologia, the European Union, QREN, FEDER, COMPETE, by funding the cE3c centre (UIDB/00329/2020), the LAQV-REQUIMTE (UIDB/50006/2020) and QOPNA (FCT UID/QUI/00062/2019) research units.en
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRosa, G.P., Barreto, M.C., Pinto, D.C.G.A. & Seca, A.M.L. (2020). A Green and simple protocol for extraction and application of a peroxidase-rich enzymatic extract. “Methods and Protocols”, 3(2), 25. DOI: 10.3390/mps3020025.en
dc.identifier.doi10.3390/mps3020025pt_PT
dc.identifier.issn2409-9279
dc.identifier.pmchttp://europepmc.org/articles/PMC7359449
dc.identifier.pmidhttps://www.ncbi.nlm.nih.gov/pubmed/32224955
dc.identifier.urihttp://hdl.handle.net/10400.3/5817
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFCT, UIDB/00329/2020pt_PT
dc.relationFCT, UIDB/50006/2020pt_PT
dc.relationFCT, UID/QUI/00062/2019pt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2409-9279/3/2/25pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBrassica rapapt_PT
dc.subjectExtractionen
dc.subjectEnzymatic Extracten
dc.subjectBiocatalysisen
dc.subjectPeroxidaseen
dc.titleA Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extracten
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceSwitzerlanden
oaire.citation.endPage12pt_PT
oaire.citation.issue(2)pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMethods and Protocolsen
oaire.citation.volume3pt_PT
person.familyNameRosa
person.familyNameBarreto
person.familyNameSeca
person.givenNameGonçalo
person.givenNameMaria do Carmo
person.givenNameAna
person.identifier.ciencia-idC512-A6FD-4A68
person.identifier.ciencia-id4518-FF32-251F
person.identifier.orcid0000-0002-0706-8505
person.identifier.orcid0000-0001-5797-8913
person.identifier.orcid0000-0002-7709-2375
person.identifier.ridN-8704-2015
person.identifier.ridE-5475-2013
person.identifier.scopus-author-id7004455600
person.identifier.scopus-author-id6603279232
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication848abdd5-db5c-4194-9b8a-4aada01290ce
relation.isAuthorOfPublicationf61a2d4d-efd3-487c-acfb-7bb7aceb55b0
relation.isAuthorOfPublication1c6b511a-fd49-4292-a8a2-b738f558eef1
relation.isAuthorOfPublication.latestForDiscovery848abdd5-db5c-4194-9b8a-4aada01290ce

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