A Green and Simple Protocol for Extraction and Application of a Peroxidase-Rich Enzymatic Extract

Rosa, Gonçalo P.; Barreto, Maria do Carmo; Pinto, Diana C. G. A.; Seca, Ana M. L.

http://hdl.handle.net/10400.3/5817

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
P1722_Seca_2020_MethodsProtocols.pdf		3.59 MB	Adobe PDF	Download

Send Feedback

Authors

Rosa, Gonçalo P.

Barreto, Maria do Carmo

Pinto, Diana C. G. A.

Seca, Ana M. L.

Abstract(s)

Recently there is a great social expectation that scientists should produce more sustainable and environmentally friendly chemical processes. Within this necessity, biocatalysis presents many attractive features because reactions are often performed in water, under mild conditions, the catalyst is biodegradable and can be obtained from renewable raw materials. In this work, we propose a simple, rapid and low-cost method for the preparation and application of an enzymatic extract from turnip root. The protocol described includes (1) the preparation of the enzymatic extract, (2) the procedure for the assessment of the more favorable working parameters (temperature, pH) and (3) the methodology for the application of the extract as the catalyst for biotransformation reactions. We anticipate that the protocol in this research will provide a simple way for obtaining an enzymatic extract which can operate efficiently under mild conditions and can effectively catalyze the biotransformation of simple phenols.

Keywords

Brassica rapa Extraction Enzymatic Extract Biocatalysis Peroxidase

URI

http://hdl.handle.net/10400.3/5817

Citation

Rosa, G.P., Barreto, M.C., Pinto, D.C.G.A. & Seca, A.M.L. (2020). A Green and simple protocol for extraction and application of a peroxidase-rich enzymatic extract. “Methods and Protocols”, 3(2), 25. DOI: 10.3390/mps3020025.