Publication
Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature : A short report
| dc.contributor.author | Paiva, Lisete S. | |
| dc.contributor.author | Lima, Elisabete | |
| dc.contributor.author | Neto, Ana I. | |
| dc.contributor.author | Baptista, José | |
| dc.date.accessioned | 2022-03-11T11:59:42Z | |
| dc.date.available | 2022-03-11T11:59:42Z | |
| dc.date.issued | 2016-11 | |
| dc.date.updated | 2022-01-28T17:54:38Z | |
| dc.description | 16th Latin-American Congress on Chromatography (COLACRO) / 9th National Meeting on Chromatography (ENC), Lisbon, PORTUGAL, JAN 05-09, 2016. | en |
| dc.description.abstract | Recently, increasing attention has been paid to the marine algae as a natural source of novel angiotensin-I converting enzyme (ACE) inhibitors, such as the phlorotannins that are the predominant polyphenols in brown algae. This study reports, for the first time, the ACE inhibition of methanol extract/fractions from Azorean brown algae Fucus spiralis (Fs) determined by HPLC-UV method, their total phenolic content (TPC) quantified as phloroglucinol equivalents (PE) and the effect of the Fs dry powder methanol extracts (Fs-DME) storage temperature on ACE inhibition. The results indicate that the ACE inhibition of Fs-DME decreased by 28.8% and 78.2% when stored during 15 days at −80 ◦C and −13 ◦C, respectively, as compared with the activity of Fs-DME at a refrigerated temperature of 6 ◦C and assayed immediately after extraction that showed a value of 80.1 ± 2.1%. This Fs-DME sample was fractionated by ultrafiltration membranes into three molecular weight ranges (3 kDa), presenting the fraction >3 kDa remarkably high ACE inhibition (88.8 ± 2.4%), TPC value (156.6 ± 1.4 mg PE/g of dry weight fraction) and yield. Furthermore, chromatographic and spectrophotometric analyses corroborate that phenolic compounds were present in Fs methanol extract/fractions, and also revealed that phloroglucinol occurs in Fs. The results seem to suggest that Azorean Fs can be a source of powerful ACE-inhibitory phlorotannins with potential impact on public health, particularly on hypertensive patients. | en |
| dc.description.sponsorship | This study was financially supported by cE3c funding (Ref: UID/BIA/00329/2013). Lisete Paiva was supported by a doctoral grant (Ref: M3.1.2/F/014/2011) awarded by FRC (Fundo Regional da Ciência). | en |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Paiva, L., Lima, E., Baptista, J., & Neto, A.I.. (2016). Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature - A short report. “Journal of Pharmaceutical and Biomedical Analysis”, 131, 503–507. https://doi.org/10.1016/j.jpba.2016.08.029 | en |
| dc.identifier.doi | 10.1016/j.jpba.2016.08.029 | pt_PT |
| dc.identifier.eissn | 1873-264X | |
| dc.identifier.issn | 0731-7085 | |
| dc.identifier.pmid | 27595387 | |
| dc.identifier.slug | cv-prod-2687102 | |
| dc.identifier.uri | http://hdl.handle.net/10400.3/6240 | |
| dc.identifier.wos | 000387628400062 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.subject | ACE-inhibitory Activity | pt_PT |
| dc.subject | HPLC | pt_PT |
| dc.subject | Hypertension | en |
| dc.subject | Phlorotannins | en |
| dc.subject | Sample Stability Studies | en |
| dc.subject | Total Phenolic Content | en |
| dc.title | Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature : A short report | en |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FBIA%2F00329%2F2013/PT | |
| oaire.citation.conferencePlace | Netherlands | en |
| oaire.citation.endPage | 507 | pt_PT |
| oaire.citation.startPage | 503 | pt_PT |
| oaire.citation.title | Journal of Pharmaceutical and Biomedical Analysis | en |
| oaire.citation.volume | 131 | pt_PT |
| oaire.fundingStream | 5876 | |
| person.familyName | Neto | |
| person.givenName | Ana | |
| person.identifier.ciencia-id | F412-5515-7324 | |
| person.identifier.orcid | 0000-0003-0708-5636 | |
| person.identifier.rid | C-1505-2010 | |
| person.identifier.scopus-author-id | 7102618919 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.cv.cienciaid | F015-95D2-365B | Lisete Sousa Paiva | |
| rcaap.rights | closedAccess | pt_PT |
| rcaap.type | article | pt_PT |
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