Recovery of lactase enzyme from calf intestine

Tavares, Joaquim F. P.

http://hdl.handle.net/10400.3/4505

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Authors

Tavares, Joaquim F. P.

Abstract(s)

The lactase from calf intestine was studied in 5 % lactose solution, inoculating 4.0 ml or 4.9 ml aliquot portions of this diluted sugar, tempered at 37˚ C in water bath, with 1.0 ml or 0.1 ml enzyme extract. The reaction monitored by analysing the glucose formed from hydrolysis of lactose by lactase present was tested, at different incubation times after inoculation with enzyme, using a YSI Model 27 Enzyme glucose Analyser. Because no recent investigations about the variations in the lactase, along the whole calf small intestine, has been done, the determination of its content, in each 10 cm intestinal fragment from stomach, was carried out. In order to estimate the best conditions, in terms of pH for separating and purifying lactase from crude liquors, using an insoluble matrix of DEAE-Cellulose in small batches, an adsorption procedure was evaluated.