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A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales

2017Journal article Restricted access
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dc.contributor.authorEmerson, Brent C.
dc.contributor.authorCasquet, Juliane
dc.contributor.authorLópez, Heriberto
dc.contributor.authorCardoso, Pedro
dc.contributor.authorBorges, Paulo A. V.
dc.contributor.authorMollaret, Noémy
dc.contributor.authorOromí, Pedro
dc.contributor.authorStrasberg, Dominique
dc.contributor.authorThébaud, Christophe
dc.date.accessioned2018-06-25T11:36:31Z
dc.date.available2018-06-25T11:36:31Z
dc.date.issued2017
dc.description.abstractObtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of R eunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly.en
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEmerson, B.C., Casquet, J., López, H., Cardoso, P., Borges, P.A.V., Mollaret, N., Oromí, P., Strasberg, D. & Thébaud, C. (2017). A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales. "Molecular Ecology Resources", 17(4): 694-707. DOI:10.1111/1755-0998.12617pt_PT
dc.identifier.doi10.1111/1755-0998.12617pt_PT
dc.identifier.issn1755-0998
dc.identifier.urihttp://hdl.handle.net/10400.3/4742
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationISLAND-BIODIV - Understanding biodiversity dynamics in tropical and subtropical islands as an aid to science based conservation action
dc.subjectBeta Diversityen
dc.subjectBiological Speciesen
dc.subjectParataxonomyen
dc.subjectSpideren
dc.subjectTaxonomyen
dc.titleA combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scalesen
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleISLAND-BIODIV - Understanding biodiversity dynamics in tropical and subtropical islands as an aid to science based conservation action
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/NETBIOME%2F0003%2F2011/PT
oaire.citation.endPage707pt_PT
oaire.citation.issue(4)pt_PT
oaire.citation.startPage694pt_PT
oaire.citation.titleMolecular Ecology Resourcespt_PT
oaire.citation.volume17pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication9ded2dc1-9e36-4fcb-9431-9acd2206153a
relation.isProjectOfPublication.latestForDiscovery9ded2dc1-9e36-4fcb-9431-9acd2206153a

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