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IITAA - Instituto de Investigação e Tecnologias Agrárias e do Ambiente

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  • Addressing Quality, Safety, and Sustainability Challenges in Artisanal Pico Cheese Production: Proteolysis Indexes, Staphylococci, and Whey Valorization
    Publication . Câmara, Sandra P. A.; Dias, Cristiana Maduro; Nunes, Hélder P. B.; Martin, Raphael; Pimentel, Francisca; Gomes, Júlia V.; Silveira, Maria da Graça A.; Rosa, Henrique J. D.; Dapkevicius, Airidas; Borba, Alfredo E. S.; Dapkevicius, Maria de Lurdes N. E.
    ABSTRACT: Artisanal cheeses face unique challenges due to changes in the present approaches to food safety, health, and environmental sustainability. This work aims at tackling such challenges in Pico cheese, by addressing outdated PDO criteria, the need to tackle coagulase-positive staphylococci (CoPS) and to promote circular economy by upgrading cheese whey. Model raw- and pasteurized milk cheeses were prepared with autochthonous lactic acid bacteria (LAB) as inoculants and analyzed for their composition, proteolysis, and microbiological parameters. CoPS were isolated and the risks they pose in terms of One Health evaluated by assessing phenotypic virulence factors and antibiotic resistance patterns. To assess the potential of autochthonous LAB for controlling CoPS, a challenge test was performed. Probiotic requeijão was prepared using autochthonous LAB as inoculants for upgrading whey. This work confirmed the need to update Pico cheese specifications regarding proteolysis indexes. Biofilm production was present in all Pico cheese CoPS, but resistance was only found against penicillin and cefoxitin. Adding salt or extending maturation time up to 60 days did not afford the desired level of CoPS control. Lactococcus lactis L1C21M1, however, was able to keep CoPS populations at 3 log cfu g−1 in the challenge test. Requeijão was a suitable substrate for probiotic autochthonous Lactococcus lactis L3A21M1 and L3B1M7.
    2025-04-24Artigo científico Acesso aberto Ver mais
  • Azorean Cryptomeria japonica immature female cones essential oil: Effect of hydrodistillation fractionation on the chemical composition and in vitro antifungal activity against Thielaviopsis paradoxa
    Publication . Arruda, Filipe; Lima, Ana; Janeiro, Alexandre; Rodrigues, Tânia; Baptista, José; Rosa, José S.; Machado, Alexandra; Figueiredo, Ana C.; Lima, Elisabete
    ABSTRACT: Cryptomeria japonica’s wood production in Azores generates large amounts of underutilized biomass residues, such as immature female cones (Az–CJIFC), that can be used to produce essential oils (EOs). Hydrodistillation (HD) can be used both to obtain and to fractionate EOs. In this study, EOs from Az–CJIFC, grinded (GR) and non grinded (NGr) fresh samples, were obtained via HD over 4 h, yielding 1.0 % and 0.5 %, w/w, respectively. Thus, GR Az–CJIFC was chosen to obtain six EO fractions (Frs. 1–6), collected at sequential HD timeframes (HDTs: 0–2, 2–10, 10–30, 30–60, 60–120, and 120–240 min). The obtained EO samples (crude EOs and fractions) were evaluated for their chemical composition (GC–FID/GC–MS analyses) and antifungal activity (micro-atmosphere method) against phytopathogenic fungi (Penicillium italicum, P. digitatum and Thielaviopsis paradoxa). Results indicated that all samples were active only towards T. paradoxa, however, with a differential efficacy, due to their specific composition. Fraction 4 vapor treatment displayed the strongest activity, but lower than that of (–)-terpinen-4-ol, a key oxygen-containing monoterpene (OCM) of Az–CJIFC EO, peaking its concentration in Fr3 (14.5 %) and Fr4 (13.8 %). This latter fraction was the richest in the OCM α-terpineol (a minor Az–CJIFC EOs’ component). On the other hand, Frs.1–3 and EOs were dominated by monoterpene hydrocarbons (65.0–96.5 %), mainly α-pinene (19.0–28.4 %) and sabinene (19.9–50.5 %), while Frs.5 and 6 were the richest in oxygen containing sesquiterpenes (47.1–70.8 %; chiefly elemol plus α-, β- and γ-eudesmol) and diterpene hydrocar bons (5.2–6.4 %; mostly phyllocladene). In conclusion, new high value-added products can now be targeted in Az–CJIFC EO by adjusting the HDT, with potential importance in pineapple fruit black rot disease management caused by T. paradoxa on Ananas comosus in the Azores, and also contributing for the local C. japonica’s EO industry development and sustainable circular bio-economy
    2025-05-17Artigo científico Acesso aberto Ver mais