Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.3/2747
Título: Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
Autor: Ferin, Rita
Pavão, Maria Leonor
Baptista, José
Palavras-chave: Ascorbic Acid
Uric Acid
Plasma Antioxidants
Data: Mai-2013
Editora: Elsevier
Citação: FERIN, R., PAVÃO, M.L. e BAPTISTA, J. (2013). "Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV", Clinical Biochemistry, 46(7-8): 665-669. http://dx.doi.org/10.1016/j.clinbiochem.2013.01.006.
Resumo: OBJECTIVES: Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. METHODS: Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase. RESULTS: Results indicated an excellent linearity with correlation coefficients (r²) ≥ 0.999. The LOD of AA and UA was 1.02 and 1.42 nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426 nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA. CONCLUSION: This validated method allows the determination of both antioxidants within 10 min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects.
Descrição: Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10400.3/2747
ISSN: 0009-9120 (Print)
1873-2933 (Online)
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S000991201300012X
Aparece nas colecções:DCFQE - Artigos em Revistas Internacionais / Articles in International Journals

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