Publication
Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
| dc.contributor.author | Ferin, Rita | |
| dc.contributor.author | Pavão, Maria Leonor | |
| dc.contributor.author | Baptista, José | |
| dc.date.accessioned | 2014-02-11T15:34:28Z | |
| dc.date.available | 2014-02-11T15:34:28Z | |
| dc.date.issued | 2013-05 | |
| dc.description | Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. | en |
| dc.description.abstract | OBJECTIVES: Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. METHODS: Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase. RESULTS: Results indicated an excellent linearity with correlation coefficients (r²) ≥ 0.999. The LOD of AA and UA was 1.02 and 1.42 nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426 nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA. CONCLUSION: This validated method allows the determination of both antioxidants within 10 min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects. | en |
| dc.description.sponsorship | Governo Regional dos Açores. | por |
| dc.identifier.citation | FERIN, R., PAVÃO, M.L. e BAPTISTA, J. (2013). "Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV", Clinical Biochemistry, 46(7-8): 665-669. http://dx.doi.org/10.1016/j.clinbiochem.2013.01.006. | en |
| dc.identifier.issn | 0009-9120 (Print) | |
| dc.identifier.issn | 1873-2933 (Online) | |
| dc.identifier.uri | http://hdl.handle.net/10400.3/2747 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S000991201300012X | por |
| dc.subject | Ascorbic Acid | en |
| dc.subject | Uric Acid | en |
| dc.subject | Plasma Antioxidants | en |
| dc.subject | HPLC-UV | por |
| dc.subject | Ion-exclusion | en |
| dc.subject | Atherosclerosis | en |
| dc.title | Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV | en |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/BD/34322/2007 | |
| oaire.citation.endPage | 669 | por |
| oaire.citation.issue | (7-8) | por |
| oaire.citation.startPage | 665 | por |
| oaire.citation.title | Clinical Biochemistry | en |
| oaire.citation.volume | 46 | por |
| oaire.fundingStream | SFRH | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
| relation.isProjectOfPublication | 973f057e-55a9-40d1-8da7-d47d5b3eb668 | |
| relation.isProjectOfPublication.latestForDiscovery | 973f057e-55a9-40d1-8da7-d47d5b3eb668 |