Logo do repositório
 
Publicação

Novelty, diversity, and genetic dark matter in enterococci of invertebrates

2026-08-20Artigo de investigação Acesso aberto
Ver registo simples
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg15:Proteger a Vida Terrestre
dc.contributor.authorSgardioli, Bruna
dc.contributor.authorPhillips, Matthew
dc.contributor.authorMiklos, Arjun
dc.contributor.authorFleiszig-Evans, Kailey
dc.contributor.authorManson, Abigail
dc.contributor.authorMello, Suelen
dc.contributor.authorShea, Terrance
dc.contributor.authorUrhan, Aysun
dc.contributor.authorWhipple, Ryan
dc.contributor.authorSalamzade, Rauf
dc.contributor.authorSanders, Jasper
dc.contributor.authorBorges, P.A.V.
dc.contributor.authorDapkevicius, Maria de Lurdes
dc.contributor.authorEarl, Ashlee
dc.contributor.authorGilmore, Michael
dc.date.accessioned2026-09-16T10:53:04Z
dc.date.available2026-09-16T10:53:04Z
dc.date.issued2026-08-20
dc.description.abstractABSTRACT: Enterococci appear to have originated in the guts of early terrestrializing arthropods and invertebrates over 425 million years ago—hosts that are now highly diverse and widespread in nature today. Yet most knowledge of the genus comes from human infection-associated lineages with genomes swollen by the recent accretion of foreign DNA conveyed by mobile elements. Because invertebrates dominate terrestrial animal diversity and biomass, they would be predicted to constitute a major but little-explored reservoir of enterococcal diversity. We therefore systematically examined Enterococcus association and species diversification in invertebrate hosts of the comparatively natural, isolated, but well-characterized environment of the Azorean island of Terceira. Over 100 invertebrate specimens were examined for associated enterococci, which were taxonomically classified by whole-genome sequencing. Supporting the existence of a large pool of uncharacterized enterococci and Enterococcus-adapted genes, 40% (eight of 20) of the Enterococcus species identified were either undescribed, including four candidate new species described here, or very recently discovered. In contrast, control isolates from vertebrates were exclusively of known species typical of sampling elsewhere, discounting geographic isolation as a main driver of the novelty observed. Further, because of the abundance of E. casseliflavus and E. flavescens in this collection, we obtained the resolution necessary to quantify the divergence and decipher the drivers of speciation in the controversial division between these naturally vancomycin-resistant species. These findings provide robust support for the existence of a large pool of new species and unexplored adaptive traits in invertebrate-associated enterococci—diverse environmental survival traits optimized for expression in an enterococcal background, and well positioned for transmission into human-associated enterococcal strains.eng
dc.identifier.citationSgardioli, B. F., Phillips, M. C., Miklos, A., Fleiszig-Evans, K., Manson, A. L., de Mello, S. S., Shea, T., Urhan, A., Whipple, R., Salamzade, R., Sanders, J., Borges, P. A. V., Dapkevicius, M. de L.N.E., Earl, A. M. & Gilmore, S. M. (2026). Novelty, diversity, and genetic dark matter in enterococci of invertebrates. mBio, e01234-26.
dc.identifier.doi10.1128/mbio.01234-26
dc.identifier.eissn2150-7511
dc.identifier.issn2150-7511
dc.identifier.urihttp://hdl.handle.net/10400.3/9046
dc.language.isoeng
dc.peerreviewedyes
dc.publisherAmercian Society Microbiology
dc.relationNational Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, by grant P01AI 083214
dc.relationgrants U19AI110818 to the Broad Institute
dc.relationNIH T32 AI007061
dc.relationIITAA was funded by FCT/MCTES through grant nos. UID/CTV/00153/2019, UIDB/00153/2020, and UID/00153/2023
dc.relation.hasversionhttps://journals.asm.org/doi/10.1128/mbio.01234-26
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectEnterococcus
dc.subjecthost association
dc.subjectinvertebrate microbiome
dc.subjectvertebrate microbiome
dc.subjectantibiotic resistance
dc.subjectgenomics
dc.subjectevolutionary biology
dc.subjectmicrobial ecology
dc.titleNovelty, diversity, and genetic dark matter in enterococci of invertebrateseng
dc.typeresearch article
dcterms.referenceshttps://journals.asm.org/doi/10.1128/mbio.01234-26
dspace.entity.typePublication
oaire.citation.startPagee01234-26
oaire.citation.titlemBio
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBorges
person.givenNamePaulo
person.identifier829215
person.identifier.ciencia-idFA1A-C9CB-9C29
person.identifier.orcid0000-0002-8448-7623
person.identifier.ridB-2780-2008
person.identifier.scopus-author-id7003533390
relation.isAuthorOfPublicationd9716a90-cc3e-44d0-adc1-6933e3786278
relation.isAuthorOfPublication.latestForDiscoveryd9716a90-cc3e-44d0-adc1-6933e3786278

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
P3011_Sgardioli_2026_mBio.pdf
Tamanho:
3.95 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.73 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

GBA - Artigos em Revistas Internacionais / Articles in International Journals