Identification of Gylptapanteles sp. (Hymenoptera - Braconidae) from Madeira island by enzymatic analysis.

Oliveira, Luísa; Vieira, Virgílio; Tavares, João; Garcia, Patrícia

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Abstract(s)

During the Scientific Expedition effectuated in Madeira Island in September 1997, Pseudaletia (Mythimna) unipuncta (Lepidoptera: Noctuidae) larvae were collected in maize fields and pastures. These larvae were parasilized by a braconid belonging to Glyptapanteles genus. This population was characterised biochemically to identify the species. Seven enzyme systems studied by electrophoresis were analysed: aldehyde oxidase (AO), aglycerophosphate dehydrogenase (a-GPO), tetrazolium oxidase (TO), malate dehydrogenase (MOH), glucose-6-dehydrogenase (G6PO), malic enzyme (ME), isocitrate dehydrogenase (IOH). All systems presented only one band, with two exceptions: a-GPO and ME with two bands which corresponded to different locus. No polimorphic enzymes were detected. Comparing this result s with those obtained from G. militaris collected in Azores Islands and in Quebec-Canada we cau suggest that the population of Glyptapanteles collected in Madeira Island belongs to G. militaris species.

Description

III Simpósio Fauna e Flora das Ilhas Atlânticas / Symposium Fauna and Flora of the Atlantic Islands, Universidade dos Açores, Ponta Delgada (Açores), 21 - 25 September, 1998.

Keywords

Electrophoresis Glyptapanteles genus Madeira Island Pseudaletia (Mythimna) unipuncta Scientific Expedition Seven Enzyme Systems

URI

http://hdl.handle.net/10400.3/1217

Citation

Oliveira, L., V. Vieira, J. Tavares & P. Garcia (1998). Identification of Gylptapanteles sp. (Hymenoptera - Braconidae) from Madeira island by enzymatic analysis. In TAVARES, J. (Ed.). Programme, Abstracts and Participants, III Symposium Fauna and Flora of the Atlantic Islands. Universidade dos Açores, Ponta Delgada (Açores), 21 - 25 September 1998, p 80.