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Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue?

2020-06Journal article Open access
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dc.contributor.authorSilva, Vanessa
dc.contributor.authorPeixoto, Fernando
dc.contributor.authorParelho, Carolina Paula Furtado de Medeiros
dc.contributor.authorGarcia, Patrícia
dc.contributor.authorRodrigues, Armindo
dc.contributor.authorSilva, Adriana
dc.contributor.authorCarvalho, Isabel
dc.contributor.authorPereira, José Eduardo
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorPoeta, Patrícia Alexandra Curado Quintas Dinis
dc.date.accessioned2021-09-07T12:19:13Z
dc.date.available2021-09-07T12:19:13Z
dc.date.issued2020-06
dc.description.abstractAntibiotics are successful drugs used in human and animal therapy; however, they must be considered as environmental pollutants. This study aims to isolate and characterize the extended-spectrum β-lactamase (ESBL) producing Escherichia coli soil from Azores Archipelago subjected to livestock agricultural practices. Twenty-four soil samples were collected from three different pasture systems with different number of cattle heads, and from a control site. Antibiotic susceptibility method was performed by Kirby-Bauer disk diffusion method against 16 antibiotics, and the presence of genes encoding lactamases, antimicrobial resistance genes, virulence factors, and phylogenetic groups was determined by polymerase chain reaction (PCR). Nine ESBLs were recovered from the three grazing sites, and all isolates presented the beta-lactamase genes blaCTX-M-3 and blaSHV. E. coli isolates were resistance to tetracycline and streptomycin and harbored the tetB, strA, and strB genes. One isolate also showed resistance to sulfonamides, and the genes sul1 and sul2 were detected. The isolates were grouped into the following phylogenic groups: B1 (n = 6), D (n = 2), and A (n = 1). The presence of antibiotics and resistance genes in soils may be the source to the development of antimicrobial resistance, which may have negative consequences in human and animal health.en
dc.description.sponsorshipThis work was supported by the Associate Laboratory for Green Chemistry- LAQV which is financed by national funds from FCT/MCTES (UID/QUI/50006/2020). Vanessa Silva received a PhD grant from FCT (Fundação para a Ciência e Tecnologia) (SFRH/BD/137947/2018).en
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSilva, V., Peixoto, F., Parelho, C., Garcia, P., Rodrigues, A., Carvalho, I., Pereira, J.E., Igrejas, G. & Poeta P. (2020). Occurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago. An environment-health pollution issue? "International Microbiology", 23(4), 619-624. DOI:10.1007/s10123-020-00134-0en
dc.identifier.doi10.1007/s10123-020-00134-0pt_PT
dc.identifier.eissn1618-1905
dc.identifier.issn1139-6709
dc.identifier.pmid32514644
dc.identifier.urihttp://hdl.handle.net/10400.3/6034
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer - Spanish Society for Microbiologyen
dc.relationUID/QUI/50006/2020pt_PT
dc.relation.publisherversionhttps://pubmed.ncbi.nlm.nih.gov/32514644/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/pt_PT
dc.subjectCTX-Mpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectEnvironmental Pollutionen
dc.subjectExtended-spectrum beta-lactamaseen
dc.subjectLivestocken
dc.subjectSoilen
dc.titleOccurrence of ESBL-producing Escherichia coli in soils subjected to livestock grazing in Azores archipelago : an environment-health pollution issue?en
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceSpainpt_PT
oaire.citation.endPage624pt_PT
oaire.citation.issue(4)pt_PT
oaire.citation.startPage619pt_PT
oaire.citation.titleInternational Microbiologyen
oaire.citation.volume23pt_PT
person.familyNameGarcia
person.familyNamedos Santos Rodrigues
person.givenNamePatrícia
person.givenNameArmindo
person.identifier.ciencia-id941F-888A-412F
person.identifier.ciencia-id141A-9835-FBE0
person.identifier.orcid0000-0003-4490-9905
person.identifier.orcid0000-0002-9851-5429
person.identifier.ridM-7754-2013
person.identifier.scopus-author-id7201694062
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication94864ead-4016-4db1-b56e-136d294db238
relation.isAuthorOfPublication375991d8-6f81-4403-902c-000634a4cfdd
relation.isAuthorOfPublication.latestForDiscovery375991d8-6f81-4403-902c-000634a4cfdd

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