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Study of protein degradability in ruminants of several feedstuffs from the Azores using the in situ artificial fiber bag technique
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Advisor(s)
Abstract(s)
A degradabilidade da proteína de oito fontes alimentares foi determinada pela técnica dos sacos de nylon in situ. Dois carneiros fistulados foram alimentados, ao nível de ingestão de manutenção e ao nível de ingestão ad libitum tendo como dieta base um feno de média qualidade. As determinações foram feitas em duplicado de forma a obter quatro réplicas. Determinámos a velocidade de trânsito das proteínas não degradadas no rúmen (k), utilizando uma técnica apontada por UDÉN & al. (1979). Os resultados obtidos foram de k = 0.066/hora para o nível de ingestão de manutenção e de k = 0.060/hora para o nível de ingestão ad libitum. Utilizando como marcador o ADP (acid detergente fiber) determinámos o azoto insolúvel (ligado à fracção ADF) e a partir deste, calculámos a digestibilidade real das proteínas. Verificámos que os resultados da digestibilidade real foram superiores aos encontrados para a digestibilidade aparente, como já era de esperar. Os resultados de degradabilidade efectiva encontrados foram: Farinha de peixe - 23.62% (manutenção) e 3.23% (ad libitum); Luzerna verde - 77.8% (manutenção) e 69,8% (ad libitum); Bagaço de soja - 59.4% (manutenção) e 57.2% (ad libitum); Prado temporário ‘Outono' - 73.3% (manutenção) e 73.1% (ad libitum); Milho grão - 47.0% (manutenção) e 50.4% (ad libitum); Pasto permanente - 69.0% (manutenção) e 67.5% (ad libitum); Erva castelhana - 70.24% (manutenção) e 71.2% (ad libitum) e Feno - 46.2% (manutenção) e 41.7% (ad libitum).
ABSTRACT: The protein degradability of eight feedstuffs was determined by the artificial fiber bag technique. Two fistulated sheep were fed, at maintenance and at the ad libitum intake level. The basal diet was a poor quality hay. The measurements were made in duplicate to obtain four replicates. The rate of passage of undegraded protein from the rumen (k) was measured, following the UDÉN & al. (1979) technique. The results obtained were k = 0.066/hour from maintenance mtake and k = 0.060/hour from ad libitum intake. Using the ADF (acid detergent fiber) as indicator, the insoluble nitrogen (connected with the ADP fraction) was calculated, and from this, the protein true digestibility was calculated. The values of true digestibility were higher than the apparent digestibility, as expected. The results of effective protein degradability obtained, were: Fishmeal - 23.6% (maintenance) and 23.2% (ad libitum); Lucerne - 77.8% (maintenance) and 69.8% (ad libitum); Soya bean oilcake - 59.4% (maintenance) and 57.2% (ad libitum); temporary pasture 'Autumn' - 73.3% (maintenance) and 73.1% (ad libitum); Maize grain · 47.0% (maintenance) and 50.4% (ad libitum); Permanent pasture - 69.0% (maintenance) and 67.5% (ad libitum); Italian ryegrass -70.24% (maintenance) and 71.2% (ad libitum) and Hay- 46.2% (maintenance) and 41.7% (ad libitum).
ABSTRACT: The protein degradability of eight feedstuffs was determined by the artificial fiber bag technique. Two fistulated sheep were fed, at maintenance and at the ad libitum intake level. The basal diet was a poor quality hay. The measurements were made in duplicate to obtain four replicates. The rate of passage of undegraded protein from the rumen (k) was measured, following the UDÉN & al. (1979) technique. The results obtained were k = 0.066/hour from maintenance mtake and k = 0.060/hour from ad libitum intake. Using the ADF (acid detergent fiber) as indicator, the insoluble nitrogen (connected with the ADP fraction) was calculated, and from this, the protein true digestibility was calculated. The values of true digestibility were higher than the apparent digestibility, as expected. The results of effective protein degradability obtained, were: Fishmeal - 23.6% (maintenance) and 23.2% (ad libitum); Lucerne - 77.8% (maintenance) and 69.8% (ad libitum); Soya bean oilcake - 59.4% (maintenance) and 57.2% (ad libitum); temporary pasture 'Autumn' - 73.3% (maintenance) and 73.1% (ad libitum); Maize grain · 47.0% (maintenance) and 50.4% (ad libitum); Permanent pasture - 69.0% (maintenance) and 67.5% (ad libitum); Italian ryegrass -70.24% (maintenance) and 71.2% (ad libitum) and Hay- 46.2% (maintenance) and 41.7% (ad libitum).
Description
Keywords
Alimentação Animal Degradação da Proteína Feedstuffs Protein Degradability
Citation
BORBA, Alfredo E. S. & João M. R. RIBEIRO (1990). Study of protein degradability in ruminants of several feedstuffs from the Azores using the in situ artificial fiber bag technique. "Arquipélago. Boletim da Universidade dos Açores: Ciências da Natureza", 8: 1-9.