Logo do repositório
 
Publicação

Genotoxicity and Cytotoxicity Assessment of Volatile Organic Compounds in Pathology Professionals Through the Buccal Micronuclei Assay

2025-05-19Artigo de investigação Acesso aberto
Ver registo simples
datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorBaptista, Fátima
dc.contributor.authorGarcia, Patrícia
dc.contributor.authordos Santos Rodrigues, Armindo
dc.contributor.authorLadeira, Carina
dc.contributor.editorTranfo, Giovanna
dc.date.accessioned2026-01-26T10:14:28Z
dc.date.available2026-01-26T10:14:28Z
dc.date.issued2025-05-19
dc.description.abstractABSTRACT: In pathology laboratories, several volatile organic compounds (VOCs) are used, such as formaldehyde, ethanol, and xylene. These substances are recognized as genotoxic and cytotoxic, which is why their handling poses risks to human health. The buccal micronucleus (MN) cytome assay is a non-invasive, useful, and simple method to detect these effects in exposed individuals. The aim of the study was to evaluate the risk of genotoxicity and cytotoxicity of VOCs in pathology professionals of S. Miguel Island, Azores, Portugal. The study comprised two groups: exposed workers (n = 21) from the three laboratories of S. Miguel, and a reference group (n = 50), randomly chosen from other hospital services without known exposure to VOCs. The exfoliated buccal cells were auto-sampled by all the participants using a cytobrush. The samples were processed in ThinPrep®, stained with modified Feulgen with Fast Green, and visualized for MN and other nuclear anomalies (ONAs), such as karyorrhexis, pyknotic, and karyolytic cells. Results showed that VOCs have a predictive significance for MN frequency, leading to the conclusion that their exposure is an increased risk factor for the health of these professionals, approximately four times greater than in the control group.eng
dc.identifier.citationBaptista F., Garcia, P. V., Rodrigues, A. S., & Ladeira, C. (2025). Genotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei Assay. Toxics, 13, 41 DOI:10.3390/toxics13050411
dc.identifier.doi10.3390/toxics13050411
dc.identifier.eissn2305-6304
dc.identifier.urihttp://hdl.handle.net/10400.3/8802
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationcE3c – Centre for Ecology, Evolution and Environmental Changes - UIDB/00329/2020 - DOI: 10.54499/UIDB/00329/2020
dc.relationIVAR – Instituto de Investigação em Vulcanologia e Avaliação de Riscos - UIDP/00643/2020 - DOI:10.54499/UIDP/00643/2020
dc.relationH&TRC – Health & Technology Research Center (FCT/MCTES) - UIDP/05608/2020 - DOI: 10.54499/UIDP/05608/2020
dc.relationH&TRC – Health & Technology Research Center (FCT/MCTES) - UIDB/05608/2020 - DOI: 10.54499/UIDB/05608/2020
dc.relation.hasversionhttps://www.mdpi.com/2305-6304/13/5/411
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectDNA damage
dc.subjectbuccal mucosa cells
dc.subjectmicronuclei
dc.subjectvolatile organic compounds
dc.subjectpathology lab
dc.subjectoccupational exposure
dc.titleGenotoxicity and Cytotoxicity Assessment of Volatile Organic Compounds in Pathology Professionals Through the Buccal Micronuclei Assayeng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.endPage423
oaire.citation.issue5
oaire.citation.startPage411
oaire.citation.titleToxics
oaire.citation.volume13
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGarcia
person.familyNamedos Santos Rodrigues
person.givenNamePatrícia
person.givenNameArmindo
person.identifier.ciencia-id941F-888A-412F
person.identifier.ciencia-id141A-9835-FBE0
person.identifier.orcid0000-0003-4490-9905
person.identifier.orcid0000-0002-9851-5429
person.identifier.ridM-7754-2013
person.identifier.scopus-author-id7201694062
relation.isAuthorOfPublication94864ead-4016-4db1-b56e-136d294db238
relation.isAuthorOfPublication375991d8-6f81-4403-902c-000634a4cfdd
relation.isAuthorOfPublication.latestForDiscovery94864ead-4016-4db1-b56e-136d294db238

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
P2819_BATISTA_2025_Genotoxicity and cytotoxicity assessment of volatile organic compounds in pathology professionals through the buccal micronuclei Assay.pdf
Tamanho:
660.58 KB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.73 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

GBA - Artigos em Revistas Internacionais / Articles in International Journals