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Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira

2019-08Journal article Open access
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dc.contributor.authorNunes, Nuno
dc.contributor.authorRosa, Gonçalo P.
dc.contributor.authorFerraz, Sónia
dc.contributor.authorBarreto, Maria do Carmo
dc.contributor.authorCarvalho, Miguel A. A. Pinheiro de
dc.date.accessioned2021-05-31T10:37:32Z
dc.date.available2021-05-31T10:37:32Z
dc.date.issued2019-08
dc.description.abstractThree macroalgae collected at Madeira Island were included in this study to determine their potential for drug, nutraceutical, food, or supplement application. Fatty acid content was higher in Zonaria tournefortii (12.32 mg g⁻¹ dw) with 16.58% of PUFAs, eicosapentaenoic acid (C20:5 omega 3), and arachidonic acid (20:4 omega 6) having concentrations of 2.59 and 1.17%, respectively. The anti-thrombogenic and anti-atherogenicity potential was higher for Z. tournefortii due to relevant fatty acids in the biochemical composition this macroalgae. Lipid classes were assessed in the lipid extract and neutral lipids (NL) were in higher yield in Asparagopsis taxiformis (51.16%) and lower in Z. tournefortii (26.96%). The glycolipids (GL) were between 36.03 and 16.11% in Z. tournefortii and Ulva lactuca. Phospholipids (PL) fraction varied from 35.91 and 31.60% in A. taxiformis and Z. tournefortii. TLC screening identified that U. lactuca contains phytol and cholesterol in its NL, digalactosyldiacylglycerol in its GL, and cardiolipin and L-alpha-phosphatidylcholine in its PL. Zonaria tournefortii contains phytol and cholesterol in its NL classes, and the PL classes contain L-alpha-phosphatidylethanolamine and 1-(3-sn-phosphatidyl)-rac-glycerol. The macroalgae A. taxiformis revealed cholesterol in its NL fraction and the same phospholipids as Z. tournefortii in its PL fraction. ATR-FTIR analysis enabled a "fingerprint" spectra and important sulfation absorption bands were identified, revealing the functional polysaccharides within these macroalgae. Anti-cholinesterasic activity was assessed in A. taxiformis, with a low IC50 for AChE (8.92 +/- 0.43 mu g mL⁻¹) and BuChE (13.96 +/- 0.32 mu g mL⁻¹), demonstrating dual inhibitory activity, justifying the interest to identify the active principle which may be the scaffold of a novel drug.en
dc.description.sponsorshipThis work was financially supported by DemoBlueAlgae “Desenvolvimento de metodologias e optimização dos processos de cultivo e processamento de macroalgas para a indústria e economia azul” PROCiência 2020 (M1420-01-0247-FEDER000002); MACBIOBLUE “Proyecto demostrativo y de transferencia tecnológica para ayudar a las empresas a desarrollar nuevos produtos y procesos en el ambito de la Biotecnología Azul de la Macaronesia” (MAC/1.1b/ 086), program Interreg MAC 2014–2020; ARDITI - Regional Agency for the Development of Research Technology and Innovation (M14-20-09-5369-FSE-000001-Doctorate in Business; Blue Iodine II “Boost Blue economy through market uptake an innovative seaweed bioextract for iodine fortification II”, grant agreement no. 733552, H2020-SMEInst-2016-2017.en
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNunes, N., Rosa, G.P., Ferraz, S., Barreto, M.C., Pinheiro de Carvalho, M.A.A. (2020). Fatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeira. "Journal of Applied Phycology", 32, 759-771. DOI:10.1007/s10811-019-01884-9en
dc.identifier.doi10.1007/s10811-019-01884-9pt_PT
dc.identifier.issn1573-5176
dc.identifier.urihttp://hdl.handle.net/10400.13/3156
dc.identifier.urihttp://hdl.handle.net/10400.3/5948
dc.identifier.wos000534338500002
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationBoost BLUE economy trough market uptake an innovative seaweed bioextract for IODINE fortification II
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10811-019-01884-9pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSeaweedsen
dc.subjectAsparagopsis taxiformispt_PT
dc.subjectUlva lactucapt_PT
dc.subjectZonaria tournefortiipt_PT
dc.subjectLipidsen
dc.subjectPUFAspt_PT
dc.subjectMUFAspt_PT
dc.subjectSFAspt_PT
dc.titleFatty acid composition, TLC screening, ATR-FTIR analysis, anti-cholinesterase activity, and in vitro cytotoxicity to A549 tumor cell line of extracts of 3 macroalgae collected in Madeiraen
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBoost BLUE economy trough market uptake an innovative seaweed bioextract for IODINE fortification II
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/733552/EU
oaire.citation.conferencePlaceNetherlandspt_PT
oaire.citation.endPage771pt_PT
oaire.citation.startPage759pt_PT
oaire.citation.titleJournal of Applied Phycologyen
oaire.citation.volume32pt_PT
oaire.fundingStreamH2020
person.familyNameRosa
person.familyNameBarreto
person.givenNameGonçalo
person.givenNameMaria do Carmo
person.identifier.ciencia-idC512-A6FD-4A68
person.identifier.orcid0000-0002-0706-8505
person.identifier.orcid0000-0001-5797-8913
person.identifier.ridN-8704-2015
person.identifier.scopus-author-id7004455600
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication848abdd5-db5c-4194-9b8a-4aada01290ce
relation.isAuthorOfPublicationf61a2d4d-efd3-487c-acfb-7bb7aceb55b0
relation.isAuthorOfPublication.latestForDiscovery848abdd5-db5c-4194-9b8a-4aada01290ce
relation.isProjectOfPublication834a8fa4-f1bd-4dec-8201-cd195959b451
relation.isProjectOfPublication.latestForDiscovery834a8fa4-f1bd-4dec-8201-cd195959b451

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