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Caracterização molecular do Cão Barbado da Terceira
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Resumo(s)
Neste trabalho analisaram-se 257 animais da raça Cão Barbado da Terceira sendo caracterizados geneticamente, por doze microssatélites identificando-se um total de 79 alelos. A variabilidade genética foi estimada através das frequências alélicas, diversidade genética, heterozigocidade esperada e observada, probabilidade de identidade e verificação do equilíbrio de Hardy-Weinberg. Analisou-se as distâncias genéticas entre grupos pré-definidos consoante o ano de nascimento. Realizaram-se testes de paternidade comparando com os dados cedidos pelos criadores dos animais em questão, com bons resultados confirmando-se a compatibilidade com os progenitores na maioria dos casos. A população continua a manter variabilidade genética suficiente para não sofrer um processo de inbreeding, contudo deve ser dada atenção aos reprodutores a serem utilizados no futuro de forma a se conseguir uma maior heterozigocidade na raça para que haja uma evolução positiva da mesma. Desta forma os objectivos deste trabalho foram conseguidos, sendo este tipo de marcadores moleculares suficientemente informativos para esta caracterização.
ABSTRACT: In this study 257 animals from the Cão Barbado da Terceira breed were analyzed, which were genetically characterized with twelve microsatellites, with a total of 79 alleles identified. The genetic variability was estimated through the allelic frequencies, genetic diversity, expected and observed heterozygosity, identity probability and verification of the Hardy-Weinberg equilibrium. There was also the analysis of the genetic distances between groups predefined according to the year of birth, as well as paternity tests by the comparison with the data provided by the breeders. Good results were obtained and the compatibility with the parents was confirmed in most cases. The population still maintains enough genetic variability in order not to undergo inbreeding however one should pay attention to the breeders to be used in the future, in a way to obtain a higher heterozygosity so there is a positive evolution of the breed. Therefore the aims of this study were achieved, and this type of molecular markers was sufficiently informative for this characterization.
ABSTRACT: In this study 257 animals from the Cão Barbado da Terceira breed were analyzed, which were genetically characterized with twelve microsatellites, with a total of 79 alleles identified. The genetic variability was estimated through the allelic frequencies, genetic diversity, expected and observed heterozygosity, identity probability and verification of the Hardy-Weinberg equilibrium. There was also the analysis of the genetic distances between groups predefined according to the year of birth, as well as paternity tests by the comparison with the data provided by the breeders. Good results were obtained and the compatibility with the parents was confirmed in most cases. The population still maintains enough genetic variability in order not to undergo inbreeding however one should pay attention to the breeders to be used in the future, in a way to obtain a higher heterozygosity so there is a positive evolution of the breed. Therefore the aims of this study were achieved, and this type of molecular markers was sufficiently informative for this characterization.
Descrição
Dissertação de Mestrado em Engenharia Zootécnica.
Palavras-chave
Cão Barbado da Terceira Caracterização Molecular Variabilidade Genética Genetic Variability Molecular Characterization Ilha Terceira (Açores)