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- Differentiation and gene flow among island and mainland populations of the true armyworm, Pseudaletia unipuncta (Haworth) (Lepidoptera: Noctuidae)Publication . Vieira, Virgílio; Pintureau, Bernard; Tavares, João; McNeil, JeremyThe genetic structure of populations of the true armyworm, Pseudaletia unipuncta (Haworth) (Lepidoptera: Noctuidae), in the Azores archipelago was studied using polyacrylamide-gel electrophoresis. Four enzyme systems (aldehyde oxidase, esterase, phosphoglucomutase, and phosphoglucose isomerase) were examined in six populations from islands in the Azores (Santa Maria, São Miguel, Terceira, Pico, Faial, Flores) and compared with those from populations from mainland Portugal and Canada. The North American and European populations are not clearly separated from the Azorean ones. Similarly, studies of different enzyme systems (aldehyde oxidase, esterase, malic enzyme, sorbitol dehydrogenase, manose-6-phosphate isomerase, and phosphoglucomutase) over 2 years (1997 and 1998) at different times of the year (spring, summer, and autumn) and at three different altitudes (0, 250, and 500 m above sea level) on three different islands (Santa Maria, São Miguel, and Faial) uncovered no distinct differences. These results, obtained from classically used loci, suggest that there is still some gene flow between sites or that island populations have not been isolated for sufficient time to have diverged from founder populations.