DNA extraction from leaves of Vaccinium cylindraceum SMITH (Ericaceae). The use of RAPD markers to detect genetic variation. Preliminary results.

Martin-Clemente, Juan; Pereira, Maria J.; Perez-Ruiz, César

http://hdl.handle.net/10400.3/834

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Authors

Martin-Clemente, Juan

Pereira, Maria J.

Perez-Ruiz, César

Abstract(s)

Vaccinium cylindraceum Smith is an endemic Ericaceae from Azores archipelago. This species is being produced by seed germination, micropropagation and stem cuttings. The produced plants are then reintroduced in their natural but disturbed environment and used to repopulate protected areas meanwhile infested with more or less invasive exotic flora. In order to protect the genetic variability within the species, a study of genetic variation between and within populations from different islands was started. DNA extraction was achieved on fresh, dried and criopreserved leaves and the First results using the PCR technique are also presented.

Keywords

DNA Extraction Genetic Vaccinium cylindraceum Smith (Ericacaeae) Azores Archipelago

URI

http://hdl.handle.net/10400.3/834

Citation

MARTÍN-CLEMENTE, J., M.J. PEREIRA & C. PEREZ-RUIZ, (2001). DNA extraction from leaves of Vaccinium cylindraceum SMITH (Ericaceae). The use of RAPD markers to detect genetic variation. Preliminary results. “Arquipélago. Life and Marine Sciences”, Supl. 2(B): 105-109.