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Occurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the Azores

2005-07Journal article Restricted access
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dc.contributor.authorMartins, Tiago
dc.contributor.authorMontiel, Rafael
dc.contributor.authorMedeiros, Jorge
dc.contributor.authorOliveira, Luísa
dc.contributor.authorSimões, Nelson
dc.date.accessioned2011-03-23T13:17:40Z
dc.date.available2011-03-23T13:17:40Z
dc.date.issued2005-07
dc.descriptionCopyright © 2005 Elsevier Inc. All rights reserved.por
dc.description.abstractA nucleopolyhedrovirus (SpliMNPV-Az) was isolated from diseased larvae of Spodoptera littoralis, collected at the Island of S. Miguel in Azores. The virulence of this isolate was tested against S. littoralis larvae in laboratory. LD50 against 2nd and 3rd instars were not significantly different larvae, but both were significantly different from that against 4th instar. The complete codons sequence of SpliMNPV-Az Polh gene obtained was 750 bp (NCBI GenBank Accession No. AY600451). This sequence was compared to other 38 polyhedrin genes from NPVs and to 6 granulin genes from GVs and resulted to be identical to the sequence of a SpliMNPV previously published, thus indicating that the natural host of SpliMNPV-Az must be S. littoralis. Genetic distances estimated from restriction enzymes profiles showed SpliMNPV-Az is close to the Egyptian SpliMNPV type B, despite some degree of genetic divergence suggested by slight differences observed on PstI profile.por
dc.description.sponsorshipFundação para a Ciência e Tecnologia (FCT); Fundação Luso Americana para o Desenvolvimento (FLAD).por
dc.identifier.citationMartins, T., R. Montiel, J. Medeiros, L. Oliveira & N. Simões, 2005. Occurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the Azores. "Journal of Invertebrate Pathology", 89(3): 185-192.por
dc.identifier.issn0022-2011
dc.identifier.urihttp://hdl.handle.net/10400.3/1073
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherELSEVIERpor
dc.relationPOCTI/AGR/2309/2001; (SFRH/BPD/13256/2003)por
dc.relation.ispartofseriesJournal of Invertebrate Pathology;89(3)
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jip.2005.06.012por
dc.subjectBaculoviruspor
dc.subjectPolyhedrinpor
dc.subjectUltrastructurepor
dc.subjectSpodoptera littoralispor
dc.subjectSpodoptera littoralis nucleopolyhedrosispor
dc.subjectVirus characterizationpor
dc.subjectVirulencepor
dc.titleOccurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the Azorespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage192por
oaire.citation.startPage185por
oaire.citation.titleJournal of Invertebrate Pathologypor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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