Vegetative propagation of the Azorean endemic shrub Viburnum treleasei Gand.

Moura, Mónica; Silva, Luís

http://hdl.handle.net/10400.3/443

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Authors

Moura, Mónica

Silva, Luís

Abstract(s)

Viburnum treleasei Gand. is a threatened hermaphroditic shrub or small tree endemic to the Azores islands. In this study we aimed at defining a fast, simple and cost-efficient propagation methodology that could be used by non-skilled workers in conservation action plans. Our objective was also to produce cleaner material for initiation of in vitro cultures and to determine the effects of season, placement of cuttings in the branch, placement of vegetative buds in cuttings and forcing solutions in shoot development. It was possible to produce clean shoots from cuttings using a forcing solution with 8-hydroxyquinoline sulphate (8-HQS), 2% sucrose and no growth regulators addition. Shoot development results obtained with apical and sub-apical cuttings indicate that V. treleasei possesses apical dominance and deep endodormancy. Apical semihardwood cuttings in autumn or airlayered branches in autumn and winter with 2 or 5% (w/w) of IBA produced excellent rooting results which will allow reinforcing depleted populations of V. treleasei efficiently and at reduced costs.