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This study was undertaken to determine the change of the temperature and relative humidity in the life cycle of Ephestia kuehniella Zeller (Lep., Pyralidae) under mass production conditions. A set of 24 cages, disposed over each other (2.5 cm apart) were introduced in a climate chamber (200 cm3), for 41 days. Each cage (6720 cm3 volume) had 10000 individuals during their pre-imaginal development. The climate chamber conditions were the following: 20.33 ± 1.2°C, 63.56 ± 8.7% RH and 16L:8D (the first 25 days) and 21.4 ± 0,9 °C, 70.5 ± 15% RH and 16L:8D (the next 16 days). All cages presented a considerable temperture increase, mainly between the 25th day and the
adults emergence. The maximum change in teh termic amplitude (8°C), due to the insect, was observed in the set center cages. On the other hand, relative humidity decresed with temperture augmentation in each set, reaching 20% (center eages) and 10% (extreme cages). In a Biotecnological context, the consequences of temperature and relative humidity changes are discussed.