Browsing by Author "Matos, Valentina"
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- Lipid extractions from marine deep-sea invertebrates : modifications to the Bligh & Dyer method to increase lipid yieldsPublication . Lino, Sílvia P. P.; Solé, Berta; Matos, Valentina; Pereira, Raquel; Xavier, Joana; Santos, Ricardo S.; Colaço, AnaThe deep-sea environment is difficult to sample, and often only small quantities of samples can be obtained when using less destructive methods than dredging. When working with marine animals that are difficult to sample and with limited quantities of tissue to extract lipids, it is essential to ensure that the used method extracts the maximum possible quantity of lipids. This study evaluates the efficiency of introducing modifications to the method originally described by Bligh & Dyer (1959). This lipid extraction method is broadly used with modifications, although these usually lack proper description and evaluation of increment in lipids. In this study we consider the improvement in terms of amount of lipids extracted by changing the method. Lipid content was determined by gravimetric measurements in eight invertebrates from the deep-sea, including deep-sea hydrothermal vents animals, using three different approaches. Results show increases of 14% to 30% in lipid contents obtained from hydrothermal vent invertebrate tissues and whole animals by placing the samples in methanol for 24 hours before applying the Bligh & Dyer mixture. Efficiency of the extractions using frozen and freeze-dried samples was also compared. For large sponges, the use of lyophilized materials resulted in increases of 3 to 7 times more lipids extracted when compared with extractions using frozen samples.
- New occurrences of Corallium spp. (Octocorallia, Coralliidae) in the Central Northeast AtlanticPublication . Sampaio, Íris; Ocaña, Óscar; Tempera, Fernando; Henriques, Andreia Braga; Matos, Valentina; Porteiro, Filipe M.Corallium niobe Bayer, 1964 and Corallium tricolor (Johnson, 1899) are reported as new records from the central Northeast Atlantic. C. niobe was caught in the Azores and C. tricolor on the slope of the Irving Seamount (South Azores Seamounts). The presence of Corallium johnsoni Gray, 1860 in Azorean waters is confirmed. The sub-tropical Macaronesian islands and seamounts appear to be an important hot-spot for Corallium spp. Most colonies have been collected on seamounts and island slopes around Madeira, Canaries, Azores and the Cape Verde archipelagos. In the Azores most of these corals live below the normal depth of commercial fishing operations and are unlikely to be severely impacted by this activity.