Moujir, Laila M.Seca, Ana M. L.Araujo, LilianaSilva, Artur M. S.Barreto, Maria do Carmo2014-03-312014-03-312011-03Moujir, Laila M.; Seca, Ana M.L.; Araujo, Liliana; Silva, Artur M.S.; Barreto, M. Carmo (2011). "A new natural spiro heterocyclic compound and the cytotoxic activity of the secondary metabolites from Juniperus brevifolia leaves", Fitoterapia, 82(2), 225-229. DOI: 10.1016/j.fitote.2010.10.001.0367-326X (Print)1873-6971 (Online)http://hdl.handle.net/10400.3/2939Copyright © 2010 Elsevier B.V. All rights reserved.A new natural spiro compound 3,4-dehydrotheaspirone 1 and the known arctiol [1β,6α-dihydroxy-4(14)-eudesmene] 2 were isolated from Juniperus brevifolia. Arctiol is reported for the first time in the Juniperus genus. Their structures were established by 1D, and 2D NMR and MS spectra. Antimicrobial and cytotoxic activities of 1 and several secondary metabolites 3,4,5,6,7,8,9,10,11,12 previously isolated by our group from J. brevifolia were evaluated and some SAR has been established. The 18-hydroxydehydroabietane displayed great antiproliferative activity against cancer cell lines tested, namely HeLa, A-549 and MCF-7. Compound also presented a significant bactericidal effect against Bacillus cereus at different concentrations tested.eng3,4-DehydrotheaspironeCupressaceaeJuniperus brevifoliaDiterpenesCytotoxicityAntimicrobial Activityjournal article2014-03-27