Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.3/4742
Título: A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales
Autor: Emerson, Brent C.
Casquet, Juliane
López, Heriberto
Cardoso, Pedro
Borges, Paulo A. V.
Mollaret, Noémy
Oromí, Pedro
Strasberg, Dominique
Thébaud, Christophe
Palavras-chave: Beta Diversity
Biological Species
Data: 2017
Editora: Wiley
Citação: Emerson, B.C., Casquet, J., López, H., Cardoso, P., Borges, P.A.V., Mollaret, N., Oromí, P., Strasberg, D. & Thébaud, C. (2017). A combined field survey and molecular identification protocol for comparing forest arthropod biodiversity across spatial scales. "Molecular Ecology Resources", 17(4): 694-707. DOI:10.1111/1755-0998.12617
Resumo: Obtaining fundamental biodiversity metrics such as alpha, beta and gamma diversity for arthropods is often complicated by a lack of prior taxonomic information and/or taxonomic expertise, which can result in unreliable morphologically based estimates. We provide a set of standardized ecological and molecular sampling protocols that can be employed by researchers whose taxonomic skills may be limited, and where there may be a lack of robust a priori information regarding the regional pool of species. These protocols combine mass sampling of arthropods, classification of samples into parataxonomic units (PUs) and selective sampling of individuals for mtDNA sequencing to infer biological species. We sampled ten lowland rainforest plots located on the volcanic oceanic island of R eunion (Mascarene archipelago) for spiders, a group with limited taxonomic and distributional data for this region. We classified adults and juveniles into PUs and then demonstrated the reconciliation of these units with presumed biological species using mtDNA sequence data, ecological data and distributional data. Because our species assignment protocol is not reliant upon prior taxonomic information, or taxonomic expertise, it minimizes the problem of the Linnean shortfall to yield diversity estimates that can be directly compared across independent studies. Field sampling can be extended to other arthropod groups and habitats by adapting our field sampling protocol accordingly.
Peer review: yes
URI: http://hdl.handle.net/10400.3/4742
DOI: 10.1111/1755-0998.12617
ISSN: 1755-0998
Aparece nas colecções:DCEA - Artigos em Revistas Internacionais / Articles in International Journals

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