Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.3/155
Título: Staining techniques for ageing tope shark, Galeorhinus galeus (Linnaeus, 1758), from the Azores : a comparison based on precision analysis.
Autor: Duarte, Pedro N.
Silva, Alexandre A.
Menezes, Gui M.
Pinho, Mário Rui
Palavras-chave: Cação
Tope Shark
Galeorhinus galeus
Açores
Azores
Data: 2001
Editora: Universidade dos Açores
Citação: "ARQUIPÉLAGO. Ciências Biológicas e Marinhas". ISSN 0873-4704. Nº 18A (2001): 65-74
Relatório da Série N.º: Ciências Biológicas e Marinhas = Life and Marine Sciences;
Resumo: A vertebrae sample of tope shark, Galeorhinus galeus (Linnaeus, 1758), stratified by sizeclasses,was used to test between different staining techniques of enhancing vertebral growth ring visibility. Four techniques were tested: alizarin red, silver nitrate and cobalt nitrate staining, and the “deep-coned vertebrae” technique. The latter was discarded due to its unsuitability for tope shark vertebrae. The cobalt nitrate original protocol was modified. Upon staining, each vertebra was subject to three replicated independent readings, by a single reader. Within-reader consistency and bias of growth ring counts determination were evaluated using four different precision indices: percent of agreement (PA), average percent error (APE), coefficient of variation (V) and index of precision (D). Results indicated that the vertebrae stained with the cobalt nitrate technique showed both better optic enhancement of growth rings and higher degree of count consistency, comparatively to the alizarin red and silver stained vertebrae. Conclusively, the cobalt nitrate was the most efficient staining technique upon tope shark vertebrae, among the methods tested herein.
URI: http://hdl.handle.net/10400.3/155
ISSN: 0873-4704
Aparece nas colecções:ARQ - LMS - Número 18A

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