Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.3/1359
Título: Freshwater molluscs from volcanic areas as model organisms to assess adaptation to metal chronic pollution
Autor: Zaldibar, Beñat
Rodrigues, Armindo
Lopes, Marco
Amaral, André
Marigómez, Ionan
Soto, Manu
Palavras-chave: Volcanism
Exposure
Biomarkers
Biomarkers of Effect
Snails
Data: Dez-2006
Editora: Elsevier
Citação: "Science of the Total Environment". ISSN 0048-9697. Volume 371, Issue 1-3, December 2006, pp. 168-175(8).
Resumo: Cellular biomarkers of exposure and biological effects were measured in digestive gland of snails (Physa acuta) sampled in sites with 12 andwithout active volcanismin SãoMiguel Island (Azores).Metal content in digestive cell lysosomeswas determined by image analysis 13 after autometallography (AMG) as volume density of autometallographed black silver deposits (VvBSD). Lysosomal structural changes 14 (lysosomal volume, surface and numerical densities – VvLYS, SvLYS and NvLYS−, and surface-to-volume ratio – S/VLYS−) were 15 quantified by image analysis, after demonstration of β-glucuronidase activity, on digestive gland cryotome sections. Additional chemical 16 analyses (atomic absorption spectrophotometry) were done in the digestive gland of snails. The highestmetal concentrations were found 17 in snails from the active volcanic site, which agreed with high intralysomal VvBSD. Digestive cell lysosomes in snails inhabiting sites 18 with active volcanismresembled a typical stress situation (enlarged and less numerous lysosomes). In conclusion, the biomarkers used in 19 this work can be applied to detect changes in metal bioavailability due to chronic exposure to metals (volcanism), in combination with 20 chemical analyses.
Descrição: Copyright © 2006 Elsevier B.V. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10400.3/1359
ISSN: 0048-9697
Versão do Editor: http://dx.doi.org/10.1016/j.scitotenv.2006.09.004
Aparece nas colecções:DBIO - Artigos em Revistas Internacionais / Articles in International Journals

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