Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.3/1073
Título: Occurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the Azores
Autor: Martins, Tiago
Montiel, Rafael
Medeiros, Jorge
Oliveira, Luísa
Simões, Nelson
Palavras-chave: Baculovirus
Polyhedrin
Ultrastructure
Spodoptera littoralis
Spodoptera littoralis nucleopolyhedrosis
Virus characterization
Virulence
Data: Jul-2005
Editora: ELSEVIER
Citação: Martins, T., R. Montiel, J. Medeiros, L. Oliveira & N. Simões, 2005. Occurrence and characterization of a nucleopolyhedrovirus from Spodoptera littoralis (Lepidoptera: Noctuidae) isolated in the Azores. "Journal of Invertebrate Pathology", 89(3): 185-192.
Relatório da Série N.º: Journal of Invertebrate Pathology;89(3)
Resumo: A nucleopolyhedrovirus (SpliMNPV-Az) was isolated from diseased larvae of Spodoptera littoralis, collected at the Island of S. Miguel in Azores. The virulence of this isolate was tested against S. littoralis larvae in laboratory. LD50 against 2nd and 3rd instars were not significantly different larvae, but both were significantly different from that against 4th instar. The complete codons sequence of SpliMNPV-Az Polh gene obtained was 750 bp (NCBI GenBank Accession No. AY600451). This sequence was compared to other 38 polyhedrin genes from NPVs and to 6 granulin genes from GVs and resulted to be identical to the sequence of a SpliMNPV previously published, thus indicating that the natural host of SpliMNPV-Az must be S. littoralis. Genetic distances estimated from restriction enzymes profiles showed SpliMNPV-Az is close to the Egyptian SpliMNPV type B, despite some degree of genetic divergence suggested by slight differences observed on PstI profile.
Descrição: Copyright © 2005 Elsevier Inc. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10400.3/1073
ISSN: 0022-2011
Versão do Editor: http://dx.doi.org/10.1016/j.jip.2005.06.012
Aparece nas colecções:DBIO - Artigos em Revistas Internacionais / Articles in International Journals

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